![]() We previously developed individual-nucleotide resolution CLIP (iCLIP), which enables PCR amplification of truncated cDNAs, and thereby identifies protein–RNA crosslink sites with nucleotide resolution ( Fig. However, in over 80% of cases, the reverse transcriptase stalls at the short polypeptide left at the UV-induced crosslink site, resulting in truncated cDNAs that lack the 5′ adapter, and are therefore not amplified in CLIP. In the original CLIP approach, reverse transcription needs to proceed from a universal 3′ ligated adapter to a universal 5′ ligated adapter, since both adapters are required for PCR amplification. Combined with high-throughput sequencing, CLIP became the standard tool for the genome-wide analysis of protein–RNA interactions. Development of in vivo UV-crosslinking and immunoprecipitation (CLIP) enabled the study of protein–RNA interactions with high positional resolution and specificity. However, these approaches were prone to identifying non-physiologic or indirect interactions and their low resolution made it difficult to narrow down actual binding sites. The first approaches to investigate protein–RNA complexes in vivo employed affinity purification or immunoprecipitation combined with microarray analysis (RIP-CHIP). In particular, RBPs cooperate and compete when binding to RNA therefore it is crucial to study protein–RNA interactions in the cellular environment. Although many RBDs recognize RNA in a sequence-specific manner, sequence information is not sufficient to reliably predict RBP binding sites throughout the transcriptome. Their RNA binding is mediated by modular RNA-binding domains (RBDs), such as the RNA recognition motif (RRM), hnRNP K-homology domain or zinc fingers (Znf). RNA-binding proteins (RBPs) are the primary regulatory factors of the various post-translational stages, including alternative splicing, polyadenylation, mRNA localization, translation and degradation. ![]() Sip a cup of tea and enter the game world or enjoy the sweetest candies with our cooking game right now.Post-transcriptional regulation critically contributes to the ability of cells to adjust gene expression in the face of a changing external or internal environment. Choose the one-player, two-player or multiplayer game and start your journey to the future world that you have never explored. Miniclip games are always proud to be one of the world-famous game sites and have the richest content through different topics. Instead, spend your free time relaxing or exploring the useful lessons of your age-appropriate game. You do not need to lose money to upgrade or join the game. In addition, your trust in our website will help you discover many new and free games. You can get ready for the new game space in the online gaming world. With the content of an open world, players achieve the best performance and show their skills. In addition, players can fully collect gaming tips and discover content quickly without being bothered. You may also be able to help a lot of other players in each of their special sections. Are you ready to top the list of the best players of all time? Take your own experience when joining new games after each game. What's even more amazing is that players are willing to share our free website with friends and open competitions to overcome numerous challenges with the highest score. The matches will make players overwhelmed by great scenery like the marvelous 3D world or interesting lessons that you can draw from the puzzle games in Miniclip unblocked games. With a knowledgeable and dynamic staff, we work non-stop to help the game site get richer with various content of many new games that any website cannot get. All of them are updated with the latest games on each daily list. You will relax with games of many interesting topics like action, driving, sports, puzzles. The benefits of playing games on our website are always a top priority. With any age and career player, when choosing games at Miniclip, they can completely master this new world without being bothered or feeling inconvenient. Miniclip Games - Free Online Games for All Ages!
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